Work Description

Title: Raw microscopy data for Wolbachia infection of JW18 cells Open Access Deposited

Attribute	Value
Methodology	Wolbachia isolated from infected JW18 cells were stained with a 1:1 mixture of SYTO9 (ThermoFisher) and Propidium Iodide (ThermoFisher) either immediately following isolation (live) or after storage at -20°C for at least 3 days prior to imaging (dead). Live Wolbachia were used straight away, without any long incubations and kept away from light prior to imaging using the same magnification, intensities, and exposure times. Microscopy and Analysis of Images All images were obtained using a Nikon Ti2 Eclipse inverted microscope at 100x magnification with a Nikon DS-Qi2 camera attachment. As noted above, cells were seeded at a density no greater than 2000 cells per well; this allowed us to more clearly delineate individual cells avoid cells clumps during the infection and made the image processing more smooth. Intensities and exposure times were as follows: green/FITC for 200ms with SOLA pad at 4%, red/Texas Red for 400ms with SOLA pad at 8%. All Z stacks were taken with a step size of 0.5μm with between 100-300 contiguous planes imaged per field, with identical settings applied across all fields, regardless of treatment and condition.
Depositor	iusw@iu.edu
Citations to related material
Resource type	Image
Last modified	06/20/2024
Language	en
Subject	Infection Drosophila Microscopy Wolbachia
Related url	http://purl.dlib.indiana.edu/iusw/data/2022/28582/Raw_Images.tgz
License	https://creativecommons.org/licenses/by-nc/4.0/

To Cite this Work:
Raw microscopy data for Wolbachia infection of JW18 cells [Data set]. Indiana University - DataCORE.