Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite

Work Description

Attribute	Value
Abstract	Traditional bottom-up mass spectrometry-based proteomics relies on the use of an enzyme, often trypsin, to generate small peptides (typically < 25 amino acids long). In top-down proteomics, proteins remain intact and are directly measured within the mass spectrometer. This technique, while inherently simpler than bottom-up proteomics, generates data which must be processed and analyzed using software tools “purpose-built” for the job. In this chapter, we will show the analysis of proteins from deconvolution and deisotoping through analysis with ProSight Lite, a free, vendor agnostic tool for the analysis of top-down mass spectrometry data. We will illustrate with two examples of intact protein spectra and discuss the iterative use of the software to characterize proteoforms and to discover the sites of post-translational modifications.
Description	Accompanying dataset to the protocol published in Methods in Molecular Biology. Click on the PURL link below in the "External Files" section to download the dataset.
Depositor	iusw@iu.edu
Citations to related material
Publisher	Methods in Molecular Biology, Springer
Resource type	Dataset
Last modified	06/20/2024
Language	en_US
Subject	Top-down, Mass spectrometry, Proteomics, ProSight Lite, Intact protein, Bioinformatics
License	http://creativecommons.org/licenses/by/4.0/
Date issued	2016
Description sponsorship	NIH/NIGMS P41GM108569

To Cite this Work:
Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite [Data set]. Indiana University - DataCORE.

Thumbnail	Title	Original Upload	Last Modified	File Size	Access	Actions
	BioinformaticsAnalysisofTopDownM...e.zip	2024-07-19	2024-07-19		Open Access	View Details Download
	README.txt	2024-08-16	2024-08-16	1.31 KB	Open Access	View Details Download

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