Work Description

Title: Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer

Open Access Deposited
http://creativecommons.org/licenses/by/4.0/
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Abstract
  • Over the past decade, developments in high resolution mass spectrometry have enabled the high throughput analysis of intact proteins from complex proteomes, leading to the identification of thousands of proteoforms. Traditionally, top-down proteomics experiments relied on hybrid ion trap – Fourier transform mass spectrometers combined with data-dependent acquisition strategies. Here, we used a benchtop quadrupole – Orbitrap instrument coupled with unique data acquisition software to identify and characterize nearly 2,000 proteoforms at 1% false discovery rate from human fibroblasts.
Methodology
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Contact information
Funding agency
  • Other Funding Agency
Other Funding agency
  • National Institute of General Medical Sciences of the National Institutes of Health. L.F. would like to acknowledge the Swiss National Science Foundation for support of an Early Postdoc Mobility fellowship
Keyword
Date coverage
  • 2016-08
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Resource type
Last modified
  • 09/22/2020
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To Cite this Work:
Fornelli, L., Durbin, K., Fellers, R., Early, B., Greer, J., LeDuc, R., Compton, P., Kelleher, N. Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer [Data set]. Indiana University - DataCORE.

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