Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer

Fornelli, Luca; Durbin, Kenneth R; Fellers, Ryan T; Early, Bryan P; Greer, Joseph B; LeDuc, Richard D; Compton, Phillip D; Kelleher, Neil L

Work Description

Title: Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer Open Access Deposited

Attribute	Value
Abstract	Over the past decade, developments in high resolution mass spectrometry have enabled the high throughput analysis of intact proteins from complex proteomes, leading to the identification of thousands of proteoforms. Traditionally, top-down proteomics experiments relied on hybrid ion trap – Fourier transform mass spectrometers combined with data-dependent acquisition strategies. Here, we used a benchtop quadrupole – Orbitrap instrument coupled with unique data acquisition software to identify and characterize nearly 2,000 proteoforms at 1% false discovery rate from human fibroblasts.
Methodology	N/A
Description	N/A
Creator	Fornelli, Luca Durbin, Kenneth R Fellers, Ryan T Early, Bryan P Greer, Joseph B LeDuc, Richard D Compton, Phillip D Kelleher, Neil L
Depositor	marmclau@iu.edu
Contact information	iusw@iu.edu
Funding agency	Other Funding Agency
Other Funding agency	National Institute of General Medical Sciences of the National Institutes of Health. L.F. would like to acknowledge the Swiss National Science Foundation for support of an Early Postdoc Mobility fellowship
Keyword	Top-down proteomics gas phase fractionation Orbitrap quadrupole data-dependent acquisition false-discovery rate mass spectrometry proteoform AUTOPILOT medium/high
Date coverage	2016-08
Citations to related material
Publisher	IUScholarWorks
Resource type	Dataset
Last modified	09/22/2020
License	http://creativecommons.org/licenses/by/4.0/

To Cite this Work:
Fornelli, L., Durbin, K., Fellers, R., Early, B., Greer, J., LeDuc, R., Compton, P., Kelleher, N. Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer [Data set]. Indiana University - DataCORE.

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